1GXL
SMC hinge domain from T. maritima with coiled coil
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 136.400, 115.500, 68.960 |
Unit cell angles | 90.00, 93.70, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.000 |
R-factor | 0.252 * |
Rwork | 0.252 |
R-free | 0.30100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | P212121 CRYSTAL FORM |
RMSD bond length | 0.009 |
RMSD bond angle | 1.363 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.075 | 0.336 |
Number of reflections | 18320 | |
<I/σ(I)> | 16.3 | 2.9 |
Completeness [%] | 95.1 | 92.6 |
Redundancy | 5.1 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 4.6 | 19 * | pH 4.60 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium citrate | 0.1 (M) | |
2 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
3 | 1 | reservoir | iso-propanol | 30 (%) |