1GWY
Crystal structure of the water-soluble state of the pore-forming cytolysin Sticholysin II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2001-05-15 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.304, 119.735, 43.421 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 10.000 * - 1.700* |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB CODE 1IAZ |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.300 * | 1.810 |
| High resolution limit [Å] | 1.700 * | 1.710 |
| Rmerge | 0.086 | 0.151 |
| Number of reflections | 31889 | |
| <I/σ(I)> | 5 | 4.6 |
| Completeness [%] | 90.3 | 90.3 |
| Redundancy | 2.1 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 20 (mM) | pH7.0 |
| 2 | 1 | drop | protein | 6-8 (mg/ml) | |
| 3 | 1 | reservoir | lithium sulfate | 0.2 (M) | |
| 4 | 1 | reservoir | Tris | 0.1 (M) | pH7.5 |
| 5 | 1 | reservoir | PEG4000 | 25-30 (%(w/v)) |
