1GWN
The crystal structure of the core domain of RhoE/Rnd3 - a constitutively activated small G protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 59.454, 59.454, 233.024 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.300 * - 2.100 |
| R-factor | 0.183 * |
| Rwork | 0.183 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1CXZ CHAIN A |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.920 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.800 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.079 | 0.343 |
| Total number of observations | 115552 * | |
| Number of reflections | 25276 | 3592 * |
| <I/σ(I)> | 6.4 | 2.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.5 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 16 * | 0.1M HEPES PH 7.5, 10% PROPAN-2-OL, 20% PEG 4000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
| 3 | 1 | reservoir | propanol-di-ol | 10 (%) | |
| 4 | 1 | reservoir | PEG4000 | 20 (%(w/v)) |
