1GWE

Atomic resolution structure of Micrococcus Lysodeikticus catalase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1996-12-15
Detector	MARRESEARCH
Spacegroup name	P 42 21 2
Unit cell lengths	105.785, 105.785, 105.001
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.540 - 0.880
R-factor	0.089
Rwork	0.089
R-free	0.09600
Structure solution method	OTHER
RMSD bond length	0.016
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.1.11)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.536	0.890
High resolution limit [Å]	0.882	0.880
Rmerge	0.048	0.275
Number of reflections	457407
<I/σ(I)>	24.4	3.44
Completeness [%]	99.2	88.8
Redundancy	7.4	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.2		pH 5.20