1GVZ
Prostate Specific Antigen (PSA) from stallion seminal plasma
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.668, 79.104, 45.783 |
| Unit cell angles | 90.00, 98.21, 90.00 |
Refinement procedure
| Resolution | 19.650 - 1.420 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.20900 * |
| Structure solution method | MIRAS |
| RMSD bond length | 0.014 * |
| RMSD bond angle | 1.606 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.470 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.074 | 0.487 |
| Total number of observations | 490556 * | |
| Number of reflections | 48282 | |
| <I/σ(I)> | 17.9 | 2.5 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | other * | 6.5 | Carvalho, A.L., (2001) Acta Cryst., D57, 1180. * |
