1GVE
Aflatoxin aldehyde reductase (AKR7A1) from Rat Liver
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-08-15 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.540, 64.680, 112.840 |
| Unit cell angles | 90.00, 91.03, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 1.380 |
| R-factor | 0.159 |
| Rwork | 0.158 |
| R-free | 0.17800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1exb |
| RMSD bond length | 0.023 * |
| RMSD bond angle | 1.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.430 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.046 | 0.467 |
| Number of reflections | 158665 * | |
| <I/σ(I)> | 24 | 1.2 |
| Completeness [%] | 85.4 | 42.8 * |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 20 * | SITTING DROP METHOD WITH 1MICROLITRE PROTEIN (6 MG/ML) AND 1MICROLITRE WELL 20% PEG8K, 0.2M LITHIUM SULPHATE, 0.1M SODIUM CITRATE PH 5. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 20 (%) | |
| 2 | 1 | reservoir | lithium sulfate | 0.2 (M) | |
| 3 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
