1GV7
ARH-I, an angiogenin/RNase A chimera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 292 |
| Detector technology | CCD |
| Collection date | 2000-09-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 38.547, 64.199, 61.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.900 * - 2.100 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b1i |
| RMSD bond length | 0.006 |
| RMSD bond angle | 24.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.108 | 0.584 |
| Total number of observations | 53460 * | |
| Number of reflections | 8426 | |
| <I/σ(I)> | 12.2 | 2 |
| Completeness [%] | 90.0 | 89.7 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.8 | 16 * | VAPOUR DIFFUSION, 16 C 10 MG/ML PROTEIN, 15 % PEG 4000, 8 % TERT-BUTANOL, 0.3 M AMMONIUM ACETATE, 0.1 M SODIUM CITRATE BUFFER, PH 5.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | pH5.8 |
| 2 | 1 | drop | PEG4000 | 7.5 (%(w/v)) | |
| 3 | 1 | drop | tert-butyl alcohol | 4 (%(v/v)) | |
| 4 | 1 | drop | ammonium acetate | 0.15 (M) | |
| 5 | 1 | drop | sodium citrate | 0.15 (M) | |
| 6 | 1 | reservoir | PEG4000 | 15 (%(w/v)) | |
| 7 | 1 | reservoir | tert-butyl alcohol | 8 (%(v/v)) | |
| 8 | 1 | reservoir | ammonium acetate | 0.3 (M) | |
| 9 | 1 | reservoir | sodium citrate | 0.1 (M) |
