1GUA
HUMAN RAP1A, RESIDUES 1-167, DOUBLE MUTANT (E30D,K31E) COMPLEXED WITH GPPNHP AND THE RAS-BINDING-DOMAIN OF HUMAN C-RAF1, RESIDUES 51-131
Experimental procedure
Detector technology | AREA DETECTOR |
Detector | SIEMENS HI-STAR |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.500, 71.800, 100.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.000 |
R-factor | 0.22 |
Rwork | 0.220 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.940 |
Data reduction software | XDS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.000 * |
Rmerge | 0.041 * |
Number of reflections | 26720 * |
Completeness [%] | 98.0 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.6 * | 19 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 11 (%) | |
10 | 1 | drop | 5 (mM) | ||
11 | 1 | drop | calcium acetate | 20-50 (mM) | |
2 | 1 | reservoir | dithiothreitol | 2 (mM) | |
3 | 1 | reservoir | Tris-HCl | 80 (mM) | |
4 | 1 | reservoir | 5 (mM) | ||
5 | 1 | reservoir | calcium acetate | 20-50 (mM) | |
6 | 1 | drop | Rap/RafRBD | 15 (mg/ml) | |
7 | 1 | drop | PEG4000 | 11 (%) | |
8 | 1 | drop | dithiothreitol | 2 (mM) | |
9 | 1 | drop | Tris-HCl | 80 (mM) |