1GTW
crystal structure of C/EBPbeta bZip homodimer bound to a DNA fragment from the tom-1A promoter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1998-12-11 |
Detector | RIGAKU IMAGE PLATE RAXIS IV |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 100.938, 112.533, 74.349 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.870 - 1.850 |
R-factor | 0.231 |
Rwork | 0.231 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h8a |
RMSD bond length | 0.004 |
RMSD bond angle | 1.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.052 | 0.430 |
Number of reflections | 44759 | |
<I/σ(I)> | 21.0506 | 1.343 |
Completeness [%] | 95.8 | 82.9 |
Redundancy | 4.008 | 2.43 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 0.2 M POTASSIUM CHLORIDE, 0.1 M MAGNESIUM ACETATE, 10.0% W/V PEG 8000, 0.05 M SODIUM CACODYLATE CACODYLATE BUFFER PH 6.5, PROTEIN-DNA COMPLEX SOLUTION CONTAINS 0.01 M DTT AND 0.005 M NAN3, PROTEIN:DNA RATIO IS 1:1.2 |