1GSQ

THREE-DIMENSIONAL STRUCTURE, CATALYTIC PROPERTIES AND EVOLUTION OF A SIGMA CLASS GLUTATHIONE TRANSFERASE FROM SQUID, A PROGENITOR OF THE LENS-CRYSTALLINS OF CEPHALOPODS

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1993-01-11
Detector	ELECTRONICS COMPUTING TECHNOLOGIES
Spacegroup name	P 31 2 1
Unit cell lengths	72.760, 72.760, 94.630
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.400
R-factor	0.18
RMSD bond length	0.018
RMSD bond angle	0.036
Data reduction software	XENGEN
Refinement software	GPRLSA

Data quality characteristics

	Overall
High resolution limit [Å]	2.400 *
Rmerge	0.087 *
Number of reflections	11745
Completeness [%]	99.7
Redundancy	3.84

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6-8 (mg/ml)
2	1	drop	Tris-HCl	25 (mM)
3	1	drop	EDTA	1 (mM)
4	1	drop	1-(S-glutathionyl)-2,4-dinitrobenzene	2 (mM)
5	1	drop	ammonium sulfate	40 (%sat)
6	1	reservoir	ammonium sulfate	60-70 (%sat)