1GSO

GLYCINAMIDE RIBONUCLEOTIDE SYNTHETASE (GAR-SYN) FROM E. COLI.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	93
Detector technology	CCD
Collection date	1997-08-01
Detector	ADSC
Wavelength(s)	0.979419, 0.979104, 0.967642, 0.9190
Spacegroup name	P 21 21 21
Unit cell lengths	56.220, 62.420, 129.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.600
R-factor	0.209
Rwork	0.209
R-free	0.24700
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	24.700 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MADSYS
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.690
High resolution limit [Å]	1.600	1.600
Rmerge	0.043 *	0.174 *
Number of reflections	58385	7256 *
<I/σ(I)>	10	4.1
Completeness [%]	95.9	83.9
Redundancy	5.2	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	18 *	0.1M MES PH6.3, 0.2M AMMONIUM SULFATE, 26% PEG 5K MONOMETHYLETHER, HANGING DROP VAPOR DEFFUSION, vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MES	0.1 (M)
2	1	reservoir	ammonium sulfate	0.1 (M)
3	1	reservoir	PEG MME5000	24 (%)