1GSD
GLUTATHIONE TRANSFERASE A1-1 IN UNLIGANDED FORM
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Collection date | 1993-09-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.92 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.200, 94.800, 104.000 |
| Unit cell angles | 90.00, 92.50, 90.00 |
Refinement procedure
| Resolution | 7.500 - 2.500 |
| R-factor | 0.238 |
| Rwork | 0.238 |
| R-free | 0.26400 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.430 |
| Data reduction software | DENZO |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.107 |
| Total number of observations | 72511 * |
| Number of reflections | 31802 |
| Completeness [%] | 97.5 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 55 (mg/ml) | |
| 2 | 1 | drop | PEG2000 | 10-20 (%) | |
| 3 | 1 | drop | Tris-HCl | 100 (mM) | |
| 4 | 1 | drop | NaAc | 30 (mM) | |
| 5 | 1 | drop | beta-mercaptoethanol | 1 (%) | |
| 6 | 1 | reservoir | PEG2000 | 10-20 (%) | |
| 7 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 8 | 1 | reservoir | NaAc | 30 (mM) | |
| 9 | 1 | reservoir | beta-mercaptoethanol | 1 (%) |
