1GQI
Structure of Pseudomonas cellulosa alpha-D-glucuronidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-07-13 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.912, 74.682, 87.587 |
| Unit cell angles | 115.18, 93.14, 109.33 |
Refinement procedure
| Resolution | 20.000 - 1.480 |
| R-factor | 0.133 |
| Rwork | 0.133 |
| R-free | 0.16800 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.589 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.560 |
| High resolution limit [Å] | 1.480 * | 1.480 |
| Rmerge | 0.073 | 0.345 |
| Number of reflections | 236943 * | 32167 * |
| <I/σ(I)> | 7.1 | 1.6 |
| Completeness [%] | 97.6 | 90.4 |
| Redundancy | 4.2 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 20 * | 30MG/ML, 15% PEG3350, 250MM MGCL2, 5MM TRIS PH8.0, 20% ETHYLENE GLYCOL, pH 8.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 5 (mM) | pH8.0 |
| 3 | 1 | reservoir | 250 (mM) | ||
| 4 | 1 | reservoir | ethylene glycol | 20 (%(v/v)) | |
| 5 | 1 | reservoir | PEG3350 | 15 (%(w/v)) |
