1GQA

Cytochrome c' from Rhodobacter Spheriodes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1999-08-15
Detector	RIGAKU IMAGE PLATE
Spacegroup name	P 31
Unit cell lengths	48.100, 48.100, 115.798
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.000 * - 1.800
R-factor	0.20948
Rwork	0.204
R-free	0.25400 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cpq
RMSD bond length	0.019 *
RMSD bond angle	1.745 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.000	1.850
High resolution limit [Å]	1.800	1.800
Rmerge	0.100 *	0.160 *
Total number of observations	67416 *
Number of reflections	24077	2553 *
<I/σ(I)>	8.1	4.6
Completeness [%]	87.0 *	57 *
Redundancy	2.8	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5		12MG/ML, 5+5 UL HANGING DROP, NA-ACETATE 25MM PH4.5, PEG 8000 15%, pH 4.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	sodium phosphate	50 (mM)	pH4.5
3	1	reservoir	PEG8000	20 (%(w/v))
4	1	reservoir	sodium phosphate	50 (mM)	pH4.5