1GPJ

Glutamyl-tRNA Reductase from Methanopyrus kandleri

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-05-15
Detector	RIGAKU RAXIS IV++
Spacegroup name	P 21 21 2
Unit cell lengths	78.267, 98.650, 68.606
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.000 - 1.950
R-factor	0.197 *
Rwork	0.212
R-free	0.26000 *
Structure solution method	MIR
RMSD bond length	0.030 *
RMSD bond angle	3.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.700	1.960
High resolution limit [Å]	1.930	1.930
Rmerge	0.073	0.324
Number of reflections	32681
<I/σ(I)>	12	2.2
Completeness [%]	91.1	69.1
Redundancy	2.5	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	4 *	METHOD: HANGING DROP, TEMP.: 4C, PROTEIN CONCENTRATION: 9.8 MG/ML, PRECIPITANT: 30 MPD, BUFFER: 100MM HEPES PH 7.5, SALT: 200MM NACL, 200MM NACITRATE, 2MM MGCL2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9.6 (mg/ml)
2	1	reservoir		0.2 (M)
3	1	reservoir		2 (mM)
4	1	reservoir	HEPES	100 (mM)	pH7.5
5	1	reservoir	sodium citrate	0.2 (M)
6	1	reservoir	MPD	30 (%(v/v))