1GOY
HYDROLASE(ENDORIBONUCLEASE)RIBONUCLEASE BI(G SPECIFIC ENDONUCLEASE) (E.C.3.1.27.-) COMPLEXED WITH GUANOSINE-3'-PHOSPHATE (3'-GMP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 287 |
Detector | SYTNEX P21 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 111.380, 69.560, 33.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.900 * - 2.000 |
R-factor | 0.184 * |
Rwork | 0.184 |
Structure solution method | MIR |
RMSD bond length | 0.019 * |
RMSD bond angle | 2.200 * |
Phasing software | BLANC |
Refinement software | REFMAC (5.1.00) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.900 * | |
High resolution limit [Å] | 2.000 | 2.000 * |
Total number of observations | 15152 * | |
Number of reflections | 15152 * | |
Completeness [%] | 82.9 * | 34.2 * |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7 | pH 7.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12-13 (mg/ml) | |
2 | 1 | drop | PEG12000 | 12 (%) | |
3 | 1 | drop | phosphate | 25 (mM) | pH7.0 |
4 | 1 | drop | ammonium sulfate | 3 (%sat) | |
5 | 1 | reservoir | PEG12000 | 23-24 (%) | |
6 | 1 | reservoir | glycerol | 50 (mM) | pH7.0 |