1GOU

Ribonuclease Binase (G Specific Endonuclease) Unliganded Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG
Synchrotron site	EMBL/DESY, HAMBURG
Temperature [K]	287
Spacegroup name	C 1 2 1
Unit cell lengths	115.030, 33.290, 78.700
Unit cell angles	90.00, 119.08, 90.00

Refinement procedure

Resolution	10.000 - 1.650
R-factor	0.14 *
Rwork	0.140
R-free	0.17600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	2.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BLANC
Refinement software	REFMAC (5.1.00)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	1.650	1.650 *
Rmerge	0.039 *	0.130 *
Total number of observations	95418 *
Number of reflections	30591 *
Completeness [%]	96.0 *	94.1 *
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7 *		pH 9.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12-13 (mg/ml)
2	1	drop	PEG12000	12 (%)
3	1	drop	phosphate	25 (mM)	pH7.0
4	1	drop	ammonium sulfate	3 (%sat)
5	1	reservoir	PEG12000	23-24 (%)
6	1	reservoir	glycerol	50 (mM)	pH7.0