1GOQ
Thermostable xylanase I from Thermoascus aurantiacus - Room temperature xylobiose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | R-AXIS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.060, 59.560, 51.130 |
| Unit cell angles | 90.00, 110.80, 90.00 |
Refinement procedure
| Resolution | 5.000 - 1.800 |
| R-factor | 0.167 |
| Rwork | 0.167 |
| R-free | 0.21200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1gok |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.170 | 0.338 |
| Number of reflections | 20709 | |
| Completeness [%] | 86.0 | 37.9 |
| Redundancy | 6.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | HANGING DROPS CONTAINING 1:1 RATIO OF 20-30 MG/ML PROTEIN SOLUTION AND RESERVOIR SOLUTION (12 % TO 25 % PEG 6,000). THE CRYSTAL WAS SOAKED IN 0.5 M XYLOBIOSE, pH 7.00 |
