1GO8
The metzincin's methionine: PrtC M226L mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-03 |
| Detector | MSC |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 102.015, 102.015, 121.302 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| Rwork | 0.169 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PRTC WILDTYPE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.739 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.0.36) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.067 | 0.399 |
| Number of reflections | 49768 | |
| <I/σ(I)> | 14.4 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | PH 6.5, NACL |
