1GO3

Structure of an archeal homolog of the eukaryotic RNA polymerase II RPB4/RPB7 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2001-02-15
Spacegroup name	P 43
Unit cell lengths	92.391, 92.391, 91.109
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 1.750
R-factor	0.217
Rwork	0.217
R-free	0.23800
Structure solution method	MIR
RMSD bond length	0.006
RMSD bond angle	22.800 *
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	MLPHARE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.000 *	1.850
High resolution limit [Å]	1.750	1.750
Rmerge	0.092	0.234
Number of reflections	76814
<I/σ(I)>	4.1	3
Completeness [%]	99.8	99.6 *
Redundancy	3.6	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *	4 *	100 MM TRIS/ HCL 1.3-1.4 M AMMONIUM DIHYDROGEN MONOPHOSPHATE, pH 4.60

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MES	20 (mM)	pH6.5
2	1	drop		100 (mM)
3	1	drop	beta-mercaptoethanol	10 (mM)
4	1	drop	protein	20-25 (mg/ml)
5	1	reservoir		1.3-1.6 (M)
6	1	reservoir	Tris-HCl	100 (mM)	pH4.6