1GO2
Structure of Ferredoxin-NADP+ Reductase with Lys 72 replaced by Glu (K72E)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE D2AM |
| Synchrotron site | ESRF |
| Beamline | D2AM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-15 |
| Spacegroup name | P 65 |
| Unit cell lengths | 86.640, 86.640, 96.270 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.700 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1que |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.858 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.190 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.710 |
| Rmerge | 0.059 | 0.340 |
| Number of reflections | 43287 | |
| <I/σ(I)> | 10.5 | 2.2 |
| Completeness [%] | 98.0 | 98 |
| Redundancy | 4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | pH 5.00 |
