1GNY

xylan-binding module CBM15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-15
Spacegroup name	C 1 2 1
Unit cell lengths	92.150, 60.122, 33.107
Unit cell angles	90.00, 102.78, 90.00

Refinement procedure

Resolution	50.000 - 1.630
R-factor	0.15 *
Rwork	0.150
R-free	0.18000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PARTIAL MODEL (UNPUBLISHED)
RMSD bond length	0.016 *
RMSD bond angle	1.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	1.690
High resolution limit [Å]	1.630	1.630
Rmerge	0.043	0.330
Number of reflections	21535
<I/σ(I)>	29	3
Completeness [%]	98.0	80
Redundancy	3.6	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6		0.2M NH4OAC,0.1M NA CITRATE PH 5.6, 30% POLYETHYLENE GLYCOL 4000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		0.2 (M)
2	1	reservoir	sodium citrate	0.1 (M)	pH5.6
3	1	reservoir	xylohexaose	10 (mM)
4	1	reservoir	PEG4000	30 (%(w/v))