1GNU
GABA(A) receptor associated protein GABARAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-15 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.013, 55.124, 64.418 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.000 * - 1.750 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eo6 |
| RMSD bond length | 0.025 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.065 | 0.247 |
| Total number of observations | 82560 * | |
| Number of reflections | 11007 | 1540 * |
| <I/σ(I)> | 5.3 | 2.2 |
| Completeness [%] | 97.6 | 95.1 |
| Redundancy | 7.6 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 22 * | 18MG/ML GABARAP, 50MM TRIS PH8.5, 0.25M NACL,10%(W/V) PEGMONO, pH 8.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 36 (mg/ml) | |
| 2 | 1 | drop | Tris | 10 (mM) | |
| 3 | 1 | drop | 0.5 (M) | pH7.0 | |
| 4 | 1 | reservoir | Tris | 0.1 (M) | pH8.5 |
| 5 | 1 | reservoir | PEG2000MME | 20 (%(w/v)) | |
| 6 | 1 | reservoir | nickel(II) chloride hexahydrate | 10 (mM) |
