1GNE

THE THREE-DIMENSIONAL STRUCTURE OF GLUTATHIONE S-TRANSFERASE OF SCHISTOSOMA JAPONICUM FUSED WITH A CONSERVED NEUTRALIZING EPITOPE ON GP41 OF HUMAN IMMUNODEFICIENCY VIRUS TYPE 1

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	94.740, 94.740, 58.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.500
R-factor	0.219
Rwork	0.219
RMSD bond length	0.020
RMSD bond angle	1.200
Phasing software	X-PLOR
Refinement software	GPRLSA

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.117 *
Number of reflections	8485 *
Completeness [%]	86.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *	22 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	PEG3350	60 (%(w/v))
3	1	drop	potassium phosphate	50 (mM)
4	1	reservoir	PEG3350	60 (%(w/v))