1GM6

3-D STRUCTURE OF A SALIVARY LIPOCALIN FROM BOAR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Collection date	2001-01-15
Spacegroup name	P 41 21 2
Unit cell lengths	70.112, 70.112, 71.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 * - 2.130
R-factor	0.252 *
Rwork	0.254
R-free	0.28300 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ew3
RMSD bond length	0.011
RMSD bond angle	1.400 *
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.800 *	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.056	0.295 *
Number of reflections	10884
<I/σ(I)>	7.3	3
Completeness [%]	99.6	99.6
Redundancy	3.9	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5		1.95M AS, 0.1M NACI PH 5.5, 0.2M K/NA TARTRATE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.95 (M)
2	1	reservoir	sodium citrate	0.1 (M)	pH5.5
3	1	reservoir	potassium/sodium tartrate	0.2 (M)
4	1	drop	protein	3 (mg/ml)
5	1	drop		10 (mM)