1GLH
CATION BINDING TO A BACILLUS (1,3-1,4)-BETA-GLUCANASE. GEOMETRY, AFFINITY AND EFFECT ON PROTEIN STABILITY
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.220, 72.560, 49.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.198 |
| R-free | 0.22500 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.047 |
| Refinement software | PROFFT |
Data quality characteristics
| Overall | |
| Number of reflections | 13475 |
| Completeness [%] | 80.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | Keitel, T., (1991) J. Mol. Biol, 218, 703. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 70 (mg/ml) | |
| 2 | 1 | drop | 2-(N-morpholino)ethanesulfonic acid | 20 (mM) | |
| 3 | 1 | drop | 0.02 (%(w/v)) | ||
| 4 | 1 | reservoir | 2-(N-morpholino)ethanesulfonic acid | 20 (mM) | |
| 5 | 1 | reservoir | 0.02 (%(w/v)) | ||
| 6 | 1 | reservoir | PEG6000 | 13 (%(w/v)) |
