1GL2

Crystal structure of an endosomal SNARE core complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS M18X
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-05-15
Detector	MAR scanner 345 mm plate
Spacegroup name	P 1 21 1
Unit cell lengths	50.700, 41.100, 51.500
Unit cell angles	90.00, 109.40, 90.00

Refinement procedure

Resolution	15.000 * - 1.900
R-factor	0.176
Rwork	0.176
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sfc
RMSD bond length	0.004
RMSD bond angle	13.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.035	0.137
Total number of observations	71259 *
Number of reflections	14969 *
<I/σ(I)>	30.57	11.35
Completeness [%]	93.7	88.8
Redundancy	5.5	3.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4 *	20 *	PROTEIN SOLUTION: 20 MG/ML OF QUATERNARY COMPLEX IN 20MM TRIS-HCL AT PH 7.4 WELL SOLUTION: 0.1M NA-ACETATE AT PH 5.2, 2.5 M NA-FORMIAT, 15% GLYCEROL HANGING DROPS WITH 2MUL + 2 MUL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)	pH7.4
3	1	reservoir	sodium acetate	0.1 (M)	pH5.2
4	1	reservoir	sodium formate	2.5 (M)
5	1	reservoir	glycerol	15 (%(v/v))