1GKE

RAT TRANSTHYRETIN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU FR-D
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	P 43 21 2
Unit cell lengths	82.729, 82.729, 161.804
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.189
Rwork	0.189
R-free	0.30300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	TETRAMER OF HUMAN TRANSTHYRETIN
RMSD bond length	0.010
RMSD bond angle	25.720 *
Data reduction software	R-AXIS (II)
Data scaling software	R-AXIS (II)
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.500	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.104	0.101
Number of reflections	16584
<I/σ(I)>	6	2.1
Completeness [%]	82.0	69.2
Redundancy	2.95	1.95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5		PH 5.0, 55-65% AMMONIUM SULFATE 0.1M ACETATE BUFFER

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	55-65 (%)
2	1	reservoir	acetate	0.1 (M)