1GK8
Rubisco from Chlamydomonas reinhardtii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Collection date | 2000-06-15 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 171.375, 142.638, 124.717 |
| Unit cell angles | 90.00, 124.13, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.400 |
| R-factor | 0.149 |
| Rwork | 0.149 |
| R-free | 0.16200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8ruc |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.430 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.049 | 0.404 |
| Total number of observations | 469880 * | |
| Number of reflections | 30387 * | |
| <I/σ(I)> | 21.7 | 2.6 |
| Completeness [%] | 97.0 | 94 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 18 * | 50 MM HEPES PH 7.5, 8-12% PEG 4000, 50 MM NAHCO3, 5 MM MGCL2, 50 UM 2-CABP, 18 DEG C, 10-15 MG/ML PROTEIN |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-15 (mg/ml) | |
| 2 | 1 | reservoir | HEPES | 50 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 8-12 (%) | |
| 4 | 1 | reservoir | 50 (mM) | ||
| 5 | 1 | reservoir | 5 (mM) |
