1GK7

HUMAN VIMENTIN COIL 1A FRAGMENT (1A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-04-15
Spacegroup name	P 62 2 2
Unit cell lengths	56.770, 56.770, 58.420
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.390 - 1.400
R-factor	0.198
Rwork	0.197
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zta
RMSD bond length	0.020
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.037	0.560
Number of reflections	11440
<I/σ(I)>	17	1.9
Completeness [%]	99.7	99.6
Redundancy	10.3	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		2.0M AMMONIUM ACETATE, 10%(V/V) DIOXANE, 0.1M MES/NA, PH6.5, pH 6.50