1GJY

The X-ray structure of the Sorcin Calcium Binding Domain (SCBD) provides insight into the phosphorylation and calcium dependent processess

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Spacegroup name	C 1 2 1
Unit cell lengths	130.925, 103.847, 78.546
Unit cell angles	90.00, 117.96, 90.00

Refinement procedure

Resolution	14.000 - 2.200
R-factor	0.223 *
Rwork	0.232
R-free	0.29600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000
High resolution limit [Å]	2.200 *
Rmerge	0.036
Number of reflections	58335
Completeness [%]	91.3 *
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.7	293 *	pH 5.70

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7 (mg/ml)
2	1	reservoir	MES	0.1 (M)	pH5.4-5.7
3	1	reservoir	ammonium sulfate	16 (%)