1GGI

CRYSTAL STRUCTURE OF AN HIV-1 NEUTRALIZING ANTIBODY 50.1 IN COMPLEX WITH ITS V3 LOOP PEPTIDE ANTIGEN

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	130.330, 52.570, 82.040
Unit cell angles	90.00, 97.50, 90.00

Refinement procedure

Resolution	10.000 * - 2.800
R-factor	0.188
Rwork	0.188
RMSD bond length	0.018
RMSD bond angle	3.790
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Rmerge	0.100 *
Completeness [%]	81.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Fab	7.5 (mg/ml)
2	1	drop	MP1 peptide	2.5 (mg/ml)
3	1	reservoir	imidazole malate	0.2 (M)
4	1	reservoir	PEG10000	14 (%)