1GFZ
FLAVOPIRIDOL INHIBITS GLYCOGEN PHOSPHORYLASE BY BINDING AT THE INHIBITOR SITE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-06-27 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.296, 128.296, 116.377 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.750 - 2.300 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.21900 |
| Structure solution method | OTHER |
| RMSD bond length | 0.008 |
| RMSD bond angle | 25.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.750 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.412 |
| Total number of observations | 310740 * | |
| Number of reflections | 43063 | |
| <I/σ(I)> | 10.8 | 2.8 |
| Completeness [%] | 98.3 | 94 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.7 | A single native T-state GPb crystal was soaked in a solution containing 5 mM caffeine, 10 mM Bes, 0.1 mM EDTA buffer, pH 6.7 for 80 min, pH 6.70, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | enzyme | 25 (mg/ml) | |
| 2 | 1 | 1 | flavopiridol | 1 (mM) | |
| 3 | 1 | 1 | dithiothreitol | 3 (mM) | |
| 4 | 1 | 1 | Bes | 10 (mM) | |
| 5 | 1 | 1 | EDTA | 0.1 (mM) | |
| 6 | 1 | 1 | sodium azide | 0.02 (%) | |
| 7 | 1 | 1 | spermine(GPa) | 1 (mM) | or 10 mM magnesium acetate |
| 8 | 1 | 1 | glucose | 50 (mM) |
