1GD0
HUMAN MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 100 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1998-10-18 |
| Detector | WEISSENBERG |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.500, 67.500, 88.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 1.500 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mif |
| RMSD bond length | 0.013 |
| RMSD bond angle | 26.100 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (98.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.026 | 0.359 |
| Number of reflections | 56679 | |
| <I/σ(I)> | 18.7 | |
| Completeness [%] | 86.9 | 67.1 |
| Redundancy | 2.7 | 1.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | ammonium sulfate, sodium citrate, pH 5.0, vapor diffusion : hanging drop, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 100 (mg/ml) | |
| 2 | 1 | drop | beta-mercaptoethanol | 14.4 (mM) | |
| 3 | 1 | drop | PMSF | 0.1 (mM) | |
| 4 | 1 | drop | sodium citrate | 100 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 0.6-1.5 (M) | |
| 6 | 1 | reservoir | HEPES | 0.1 (M) |
