1GC6
CRYSTAL STRUCTURE OF THE RADIXIN FERM DOMAIN COMPLEXED WITH INOSITOL-(1,4,5)-TRIPHOSPHATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-C |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-03-21 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 96.800, 96.800, 132.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| Rwork | 0.229 |
| R-free | 0.28300 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.205 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.081 | 0.485 |
| Number of reflections | 14511 | |
| <I/σ(I)> | 17.9 | |
| Completeness [%] | 99.5 | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 4 * | PEG 6000, MES, INOSITOL-(1,4,5)-TRIPHOSPHATE, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | MES-Na | 50 (mM) | |
| 3 | 1 | reservoir | PEG6000 | 2 (%) | |
| 4 | 1 | reservoir | 150 (mM) | ||
| 5 | 1 | reservoir | dithiothreitol | 0.5 (mM) |
