1GBN
HUMAN ORNITHINE AMINOTRANSFERASE COMPLEXED WITH THE NEUROTOXIN GABACULINE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1995-10-10 |
| Detector | PRINCETON 2K |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 115.013, 115.013, 185.753 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.206 * |
| Rwork | 0.209 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oat |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.320 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.064 | 0.171 * |
| Number of reflections | 57010 | |
| <I/σ(I)> | 16.2 | 5.9 |
| Completeness [%] | 89.9 * | 80.1 |
| Redundancy | 4.6 | 2.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.8 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | PLP | 0.01 (M) | |
| 4 | 1 | reservoir | PEG6000 | 10-15 (%) | |
| 5 | 1 | reservoir | 50-200 (mM) |
