1GAL
CRYSTAL STRUCTURE OF GLUCOSE OXIDASE FROM ASPERGILLUS NIGER: REFINED AT 2.3 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 66.500, 66.500, 214.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.048 * |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 * | Kalisz, H.M., (1990) J.Mol.Biol., 213, 207. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | glucose oxidase | 20 (mg/ml) | |
| 2 | 1 | drop | acetate | 50 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 1.6 (M) | |
| 4 | 1 | drop | octanetriol solution | saturated | |
| 5 | 1 | reservoir | ammonium sulfate | 1.6 (M) |
