1GAK
CRYSTAL STRUCTURE OF GREEN ABALONE SP18
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.77 |
| Spacegroup name | P 65 |
| Unit cell lengths | 68.760, 68.760, 67.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.000 - 1.850 |
| R-factor | 0.227 * |
| Rwork | 0.227 |
| R-free | 0.24730 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.242 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.760 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.068 | 0.311 |
| Total number of observations | 82274 * | |
| Number of reflections | 15134 | |
| <I/σ(I)> | 8 | 2.4 |
| Completeness [%] | 98.9 | 98.9 |
| Redundancy | 5.4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.9 * | 22 * | mPEG 5000, Ammonium Acetate, Sodium Phosphate, pH 3, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium acetate | 50 (mM) | |
| 2 | 1 | drop | protein | 10 (mg/ml) | |
| 3 | 1 | reservoir | mPEG5000 | 25 (%) | |
| 4 | 1 | reservoir | ammonium acetate | 200 (mM) | |
| 5 | 1 | reservoir | sodium phosphate | 100 (mM) |
