1G9Y
HOMING ENDONUCLEASE I-CREI / DNA SUBSTRATE COMPLEX WITH CALCIUM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-05-01 |
Detector | ADSC |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.200, 67.800, 88.300 |
Unit cell angles | 90.00, 91.60, 90.00 |
Refinement procedure
Resolution | 26.990 - 2.050 |
Rwork | 0.207 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | CRE/DNA (PDB ENTRY 1BP7) |
RMSD bond length | 0.005 |
RMSD bond angle | 20.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.055 | 0.360 |
Number of reflections | 30037 | |
<I/σ(I)> | 13.1 | |
Completeness [%] | 91.4 | 81.7 |
Redundancy | 3.2 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 25% PEG 400, pH 6.5, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 400 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | 10 (mM) | ||
2 | 1 | drop | protein | 3.5 (mg/ml) | |
3 | 1 | reservoir | 20 (mM) | ||
4 | 1 | reservoir | MES | 100 (mM) | |
5 | 1 | reservoir | PEG400 | 20-35 (%(v/v)) |