1G8R
MOEA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2000-09-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.900, 98.600, 159.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 * |
| Rwork | 0.221 |
| R-free | 0.26600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g8l |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.478 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.700 * | 2.600 |
| Rmerge | 0.098 * | 0.309 * |
| Number of reflections | 34006 | |
| <I/σ(I)> | 10.7 | 1.39 |
| Completeness [%] | 98.8 * | 98.6 * |
| Redundancy | 3.7 * | 3.26 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG 6000, calcium acetate, cacodylaic acid, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 10-15 (%) | or PEG8000 |
| 2 | 1 | reservoir | calcium acetate | 0.2-0.5 (M) | |
| 3 | 1 | reservoir | cacodylic acid | 0.1 (M) |
