1G8R

MOEA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	95
Detector technology	CCD
Collection date	2000-09-30
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	68.900, 98.600, 159.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.700 *
Rwork	0.221
R-free	0.26600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g8l
RMSD bond length	0.008 *
RMSD bond angle	1.478 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.700 *	2.600
Rmerge	0.098 *	0.309 *
Number of reflections	34006
<I/σ(I)>	10.7	1.39
Completeness [%]	98.8 *	98.6 *
Redundancy	3.7 *	3.26

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	PEG 6000, calcium acetate, cacodylaic acid, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	10-15 (%)	or PEG8000
2	1	reservoir	calcium acetate	0.2-0.5 (M)
3	1	reservoir	cacodylic acid	0.1 (M)