1G62
CRYSTAL STRUCTURE OF S.CEREVISIAE EIF6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-07-04 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 56.257, 56.257, 171.703 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.500 |
| R-factor | 0.197 * |
| Rwork | 0.197 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g61 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.086 | 0.340 |
| Total number of observations | 60623 * | |
| Number of reflections | 9926 | |
| <I/σ(I)> | 15.7 | |
| Completeness [%] | 96.8 | 84.9 |
| Redundancy | 6.1 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 297 | 2M Ammonium Sulfate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 24.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 2 (M) |
