1G5N

ANNEXIN V COMPLEX WITH HEPARIN OLIGOSACCHARIDES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	90
Detector technology	CCD
Collection date	1999-09-25
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979
Spacegroup name	H 3
Unit cell lengths	155.874, 155.874, 37.344
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	100.000 - 1.900
R-factor	0.205 *
Rwork	0.208
R-free	0.23600 *
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	Unpublished structure of wild-type annexin V
RMSD bond length	0.005 *
RMSD bond angle	1.090 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.020
High resolution limit [Å]	1.900	1.900
Rmerge	0.086	0.219
Total number of observations	144885 *
Number of reflections	26655 *
<I/σ(I)>	16.5
Completeness [%]	92.1	93.5
Redundancy	7	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.2	17 *	Campos, B., (1998) Biochemistry, 37, 8004. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	14 (mg/ml)
2	1	drop	calcium	20 (mM)
3	1	reservoir	ammonium sulfate	34-40 (%sat)
4	1	reservoir	HEPPS	50 (mM)
5	1	reservoir		0.02 (%)
6	1	reservoir	dithiothreitol	2 (mM)
7	1	reservoir		10 (mM)