1G51

ASPARTYL TRNA SYNTHETASE FROM THERMUS THERMOPHILUS AT 2.4 A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1996-04-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	60.135, 155.500, 171.145
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.000 - 2.400
R-factor	0.194
Rwork	0.194
R-free	0.25100
RMSD bond length	0.013
RMSD bond angle	24.300 *
Data reduction software	XDS
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	11.500	2.450
High resolution limit [Å]	2.600	2.400
Rmerge	0.053	0.285
Number of reflections	60454
<I/σ(I)>	22.15
Completeness [%]	95.4	87.6
Redundancy	3.11	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	271	seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	sodium formate	2 (M)
3	1	drop	Tris-HCl	50 (mM)
4	1	drop	Tris-HCl	50 (mM)
5	1	reservoir	sodium formate	2 (M)