1G4M

CRYSTAL STRUCTURE OF BOVINE BETA-ARRESTIN 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2000-04-30
Detector	BRANDEIS - B4
Wavelength(s)	0.979, 1.10
Spacegroup name	P 1 21 1
Unit cell lengths	62.400, 73.717, 115.760
Unit cell angles	90.00, 98.73, 90.00

Refinement procedure

Resolution	34.540 - 1.900
R-factor	0.235
Rwork	0.235
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT, SIRAS
Starting model (for MR)	1cf1
RMSD bond length	0.011
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000 *
High resolution limit [Å]	1.960 *
Rmerge	0.047 *	0.468 *
Total number of observations	158905 *
Number of reflections	49309 *
Completeness [%]	65.8 *
Redundancy	3.2 *	1.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	8.5 *	19-20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	5 (mg/ml)	or 7mg/ml
2	1	1		0.1 (M)
3	1	1	Tris-HCl	20 (mM)
4	1	1	EDTA	1 (mM)
5	1	1	dithiothreitol	10 (mM)
6	1	1	glycerol	5 (%)
7	1	2	PEG4000	12-14.5 (%)
8	1	2		0.4 (M)
9	1	2	Tris-HCl	0.1 (M)