1G38
ADENINE-SPECIFIC METHYLTRANSFERASE M. TAQ I/DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.500, 68.650, 114.430 |
| Unit cell angles | 90.00, 92.20, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.196 * |
| Rwork | 0.196 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2adm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.130 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.088 | 0.195 |
| Total number of observations | 218047 * | |
| Number of reflections | 54457 | |
| <I/σ(I)> | 10.42 | 3.6 |
| Completeness [%] | 87.1 | 67.1 |
| Redundancy | 4 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 * | 20 * | 15% Isopropanol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | isopropanol |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | M-Taql | 0.075 (mM) | |
| 2 | 1 | drop | oligodeoxynucleotide | 0.100 (mM) | |
| 3 | 1 | drop | AETA | 2 (mM) | |
| 4 | 1 | drop | Tris-HCl | 10 (mM) | |
| 5 | 1 | drop | 300 (mM) | ||
| 6 | 1 | reservoir | sodium cacodylate | 50 (mM) | |
| 7 | 1 | reservoir | 100 (mM) | ||
| 8 | 1 | reservoir | 25 (mM) | ||
| 9 | 1 | reservoir | iso-propanol | 15 (%(v/v)) |
