1G35
CRYSTAL STRUCTURE OF HIV-1 PROTEASE IN COMPLEX WITH INHIBITOR, AHA024
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 278 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-04-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.3750 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 58.500, 86.900, 46.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| Rwork | 0.195 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ajx |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 25.000 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.096 | 0.310 |
| Number of reflections | 19437 | |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 85.1 | 80.2 |
| Redundancy | 15.3 | 1.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.5 | 277 | 50 mM MES, 0.4 M NaCl, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
