1G31

GP31 CO-CHAPERONIN FROM BACTERIOPHAGE T4

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	1997-01
Detector	PRINCETON 2K
Spacegroup name	P 42 21 2
Unit cell lengths	157.677, 157.677, 90.932
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.225
Rwork	0.225
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ESCHERICHIA COLI GROES 2.8 ANGSTROM MODEL
RMSD bond length	0.012
RMSD bond angle	25.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.108 *
Number of reflections	42831
<I/σ(I)>	10.6	1.9
Completeness [%]	83.5	65
Redundancy	5.95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7 *	21 *	PROTEIN WAS CRYSTALLIZED AT 17 MG/ML FROM 0.42 M NAH(2)PO(4), 1.70 M K(2)HPO(4), 10 MM BES, 6 MM DTT, 0.035% NAN(3), 29% ETHYLENE GLYCOL.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	17 (mg/ml)
2	1	drop	dithiothreitol	4 (mM)
3	1	drop	BES	10 (mM)
4	1	reservoir	dithiothreitol	2 (mM)
5	1	reservoir	sodium phospahte	0.42 (M)
6	1	reservoir	potassium phosphate	1.70 (M)
7	1	reservoir	ethylene glycol	29 (%(v/v))
8	1	reservoir		0.035 (%(w/v))