1G2C

HUMAN RESPIRATORY SYNCYTIAL VIRUS FUSION PROTEIN CORE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	1999-07-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9816
Spacegroup name	P 1
Unit cell lengths	67.904, 71.539, 76.455
Unit cell angles	81.34, 73.80, 60.72

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.233
Rwork	0.233
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.036	0.240
Total number of observations	119905 *
Number of reflections	56973
<I/σ(I)>	8.9
Completeness [%]	91.2	64.7
Redundancy	1.3	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	PEG 4000, Tris-HCl, Li2SO4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG4000	24-26 (%)
3	1	reservoir	Tris-HCl	200 (mM)
4	1	reservoir		300 (mM)