1G1K

COHESIN MODULE FROM THE CELLULOSOME OF CLOSTRIDIUM CELLULOLYTICUM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID2
Synchrotron site	ESRF
Beamline	ID2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-02-22
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	56.500, 57.250, 105.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.23
Rwork	0.230
R-free	0.27000
RMSD bond length	0.014
RMSD bond angle	2.000
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.060
High resolution limit [Å]	2.000	2.000
Rmerge	0.082	0.275
Number of reflections	22829
<I/σ(I)>	6.4
Completeness [%]	98.0	98.8 *
Redundancy	3.5	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	20 *	used macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG8000	30 (%(w/v))
3	1	reservoir	ammonium sulfate	150 (mM)