1G08
CARBONMONOXY LIGANDED BOVINE HEMOGLOBIN PH 5.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1991-09-01 |
| Detector | SDMS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.400, 110.900, 66.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.023 |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | MERLOT |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.690 | 2.030 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.058 | 0.232 |
| Total number of observations | 334873 * | |
| Number of reflections | 45723 | |
| <I/σ(I)> | 29.45 | |
| Completeness [%] | 96.8 | 85.3 |
| Redundancy | 7.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 5 | 296 | sodium cacodylate, sodium dithionite, polyethylene glycol 3350, pH 5.0, Batch, temperature 296K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | bovine carbonmonoxyhemoglobin | 2 (%) | |
| 2 | 1 | 1 | sodium cacodylate | 300 (mM) | |
| 3 | 1 | 1 | sodium dithionite | 2 (mM) | |
| 4 | 1 | 1 | Dow PEG3350 | 11.5 (%) |
